Plasmid

Part:BBa_K4761110

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-10-02)


pEffector

Carries the gRNA sequence and transposon-relevant genes. One of the CRISPR-Transposon three-plasmid system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2470
    Illegal XbaI site found at 756
    Illegal XbaI site found at 7016
    Illegal XbaI site found at 7411
    Illegal XbaI site found at 8033
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2470
    Illegal NheI site found at 2324
    Illegal NheI site found at 3896
    Illegal NheI site found at 5954
    Illegal NheI site found at 10075
    Illegal NheI site found at 11898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2470
    Illegal BglII site found at 3322
    Illegal BglII site found at 4113
    Illegal BglII site found at 4643
    Illegal BglII site found at 9862
    Illegal BamHI site found at 2312
    Illegal BamHI site found at 2464
    Illegal XhoI site found at 2153
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2470
    Illegal XbaI site found at 756
    Illegal XbaI site found at 7016
    Illegal XbaI site found at 7411
    Illegal XbaI site found at 8033
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2470
    Illegal XbaI site found at 756
    Illegal XbaI site found at 7016
    Illegal XbaI site found at 7411
    Illegal XbaI site found at 8033
    Illegal NgoMIV site found at 11151
    Illegal AgeI site found at 9994
    Illegal AgeI site found at 10898
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2248
    Illegal SapI.rc site found at 5399


[edit]
Categories
Parameters
None